Development and Validation of UV Spectrophotometric Method for the Estimation of Linezolid

The objective of this study was to develop and validate a sensitive, simple, accurate, precise and cost-effective UV spectrophotometric method for the quantification of Linezolid (LNZ) in pharmaceutical formulations of lipid nanoparticles. Various analytical performance parameters, including linearity, range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined in accordance with the International Conference on Harmonization (ICH) Q2 (R1) guidelines. The analysis was carried out using a mixture of 80% water and 20% methanol (v/v). The maximum absorption peak (λmax) of LNZ was found to be 251 nm in the selected medium. Beer-Lambert’s law was valid in the concentration range of 0.5–10 μg/ml, with a high correlation coefficient (R2) of 0.9955. The study results affirm that the developed procedure is both accurate and precise, with reproducible outcomes. Additionally, it is characterized by its simplicity, affordability, and time efficiency. Consequently, this method can be effectively employed for the quantification of LNZ in prepared lipid nanoparticles.